Drosophila muscle driver

 · Similarly, another muscle specific driver, cGAL4 crossed to heterozygous UAS-Raptor-shRNA/TM6B resulted in a reduced number muscle-specific Raptor knockdown flies (i.e. cGAL4 UAS-Raptor Cited by:  · Drosophila IFMs of the thorax belong to the fibrillar muscle group and are stretch-activated, oxidative, slow twitch muscles that are similar to vertebrate cardiac muscles (Schönbauer et al., ). By contrast, Drosophila leg muscles and abdominal muscles belong to the tubular.  · The development and molecular composition of muscle tissue is evolutionarily conserved. Drosophila is a powerful in vivo model system to investigate muscle morphogenesis and function. Here, we provide a short and comprehensive overview of the important developmental steps to build Drosophila body muscle in embryos, larvae and pupae. We describe key methods, including muscle Cited by:

Drosophila has a long history as a genetic model to study myogenesis, both the muscle structure and core myogenic programs being highly conserved between flies and mammals [19,20,21]. Drosophila myogenesis proceeds in two distinct waves, leading to the formation of adequate sets of muscles for the stage-specific modes of locomotion. The first wave happens during embryonic development, and it forms the body wall muscles required for larval crawling. We have generated and characterized a complementary model of MEGF10/Drpr gain-of-function in Drosophila muscle. Our MEGF10 loss of function and gain of function Drosophila models enable us to begin dissecting the conserved functional pathways regulated by this protein in muscle cells. Using our fly models, we have initiated genetic studies focused on interactions with the Notch pathway, which is an important regulator of muscle cell proliferation and differentiation. P{Mef2-GAL4} on the 3rd chromosome (B, Bloomington Drosophila stock center) served as a muscle-specific driver and was recombined with P{UAS-His2av-mKO}3 (B) to mark myonuclei with Histone H2Av-mKO (monomeric kusabira orange), henceforth referred to as histone-mKO.

1 de jul. de Here, we show that Drosophila muscle modulates obesity through the function Using a different fly muscle-specific driver, Mhc-Gal4 (22). 4 de nov. de Using a series of GAL4 drivers we also show that muscle-specific Raptor knockdown also causes shortened lifespan, even when eclosure is. 15 de jan. de In Drosophila, the adult muscle progenitors (AMPs) respond to Notch signaling in NP1-GAL4 (gut epithelial driver) and Actin-GAL4/cyo,GFP.